Y1H

Yeast One Hybrid Services Core

Y1H Core Logo

The Yeast One Hybrid Services Core provides a screening service between promoters and collections of Arabidopsis, Maize, or Sorghum transcription factors.

 

The purpose of the Yeast One Hybrid Services Core is to carry out high-throughput mating-based yeast assays to screen for protein (transcription factor) – DNA (promoter or regulatory region) interactions. These gene-centered assays start with a DNA regulatory region of interest and enable identification of transcription factor(s) that interact with this region.

SERVICES

  • Yeast One Hybrid Screening of 1-14 Promoters against 800 root-expressed Arabidopsis transcription factors
  • Yeast One Hybrid Screening of 1-6 Promoters against a complete collection of 2,000 Arabidopsis transcription factors
  • Yeast One Hybrid Screening of 1-24 Promoters against 500 Maize transcription factors
  • Yeast One Hybrid Screening of 1-35 Promoters against 182 Sorghum transcription factors
  • Yeast One Hybrid training
  • Custom screening against a full collection of 2,000 Maize transcription factors
  • Custom screening against a human transcription factor collection

 


Four collections of transcription factors are available:

i.  A root stele-expressed collection of 800 Arabidopsis transcription factors (Gaudinier, Zhang et al. 2011; Li, Gaudinier et al. 2014) TFLIST 12-10-2014 CLM-1 (-850 root expressed TFs);

ii.  A complete collection of 2,000 Arabidopsis transcription factors (Pruneda-Paz, Breton et al. 2014) TFLIST 11-30-2015 (full Arabidopsis TF collection)

iii.  A collection of 500 maize transcription factors (Burdo, Gray et al. 2014) Maize TF Master list 3-2016;

iv. A collection of 182 Sorghum transcription factors (expressed in the root with enrichment in the vasculature). Original open reading frames synthesized as part of a DOE JGI CSP call (Sorghum TF list).

 

Photo by E. GeorgianThe user will recombine their promoter(s) into a Gateway 5’TOPO plasmid (or Gateway pDONR-P4P1 plasmid if available) followed by the pMW2 and pMW3 yeast reporter plasmids. These constructs will then be provided to the UC Davis Yeast One Hybrid Services Core. The UC Davis Yeast One Hybrid Services Core completes yeast transformation, auto-activation tests, genotyping, and mating-based screens. Positive interactions are recorded for LacZ and HIS3 reporters.

 

Questions?  Please contact Siobhan Brady/sbrady@ucdavis.edu or Anne-Maarit Bagman/ambagman@ucdavis.edu

The Yeast One Hybrid Service Core is located within the Brady Lab.

 

Please see the Yeast One Hybrid Services Core's approach for authorships/collaborations for publications

 

Set up a PPMS account here   (instructions here)


Service University of California Non-Profit/ Education For-Profit/ Industry
Y1H Screening of 1-14 Promoters/Arabidopsis 800 $6,690 $10,503 $12,958
Y1H Consumables only per Promoter/Arabidopsis 800 $159 $249 $307
Y1H Training Arabidopsis 800 $5,081 $7,977 $9,841
Y1H Addt’l Screening/Arabidopsis 800 $1,847 $2,900 $3,578
Y1H Screening of 1-6 Promoters/Arabidopsis 2000 $6,883 $10,807 $13,333
Y1H Consumables only per Promoter/Arabidopsis 2000 $417 $654 $807
Y1H Training Arabidopsis 2000 $5,360 $8,415 $10,382
Y1H Addt’l Screening/Arabidopsis 2000 $2,220 $3,485 $4,300
Y1H Screening of 1-24 Promoters/Maize 500 $7,070 $11,099 $13,694
Y1H Consumables only per Promoter/Maize 500 $119 $187 $231
Y1H Training Maize 500 $5,360 $8,415 $10,382
Y1H Addt’l Screening - Trained Customer/Maize 500 $924 $1,450 $1,789
YIH Screening of 1-35 Promoters/Sorghum 182 $5,613 $8,813

$10,873

YIH Consumables only per Promoter/Sorghum 182 $90 $141 $174
YIH Training - Sorghum 182 $4,336 $6,807 $8,398
YIH Addt'l Screening - Trained Customer/Sorghum 182 $924 $1,450 $1,789
Special Labor $62 $97 $120
Special Consumables $1 $1 $1

Literature Cited

Burdo, B., J. Gray, M. P. Goetting-Minesky, B. Wittler, M. Hunt, T. Li, D. Velliquette, J. Thomas, I. Gentzel, M. dos Santos Brito, M. K. Mejia-Guerra, L. N. Connolly, D. Qaisi, W. Li, M. I. Casas, A. I. Doseff and E. Grotewold (2014). “The Maize TFome–development of a transcription factor open reading frame collection for functional genomics.” Plant J 80(2): 356-366.

Gaudinier, A., L. Zhang, J. S. Reece-Hoyes, M. Taylor-Teeples, L. Pu, Z. Liu, G. Breton, J. L. Pruneda-Paz, D. Kim, S. A. Kay, A. J. Walhout, D. Ware and S. M. Brady (2011). “Enhanced Y1H assays for Arabidopsis.” Nat Methods 8(12): 1053-1055.

Li, B., A. Gaudinier, M. Tang, M. Taylor-Teeples, N. T. Nham, C. Ghaffari, D. Benson, M. Steinmann, J. A. Gray, S. M. Brady and D. J. Kliebenstein (2014). “Promoter based integration in plant defense regulation.” Plant Physiol.

Pruneda-Paz, J. L., G. Breton, D. H. Nagel, S. E. Kang, K. Bonaldi, C. J. Doherty, S. Ravelo, M. Galli, J. R. Ecker and S. A. Kay (2014). “A genome-scale resource for the functional characterization of Arabidopsis transcription factors.” Cell Rep 8(2): 622-632.