The Yeast One Hybrid Services Core provides a screening service between promoters and collections of Arabidopsis, Maize, or Sorghum transcription factors.
The purpose of the Yeast One Hybrid Services Core is to carry out high-throughput mating-based yeast assays to screen for protein (transcription factor) – DNA (promoter or regulatory region) interactions. These gene-centered assays start with a DNA regulatory region of interest and enable identification of transcription factor(s) that interact with this region.
- Yeast One Hybrid Screening of 1-6 Promoters against a complete collection of 2,000 Arabidopsis transcription factors
- Yeast One Hybrid Screening of 1-24 Promoters against 500 Maize transcription factors
- Yeast One Hybrid Screening of 1-35 Promoters against 182 Sorghum transcription factors
- Yeast One Hybrid training
- Custom screening against a full collection of 2,000 Maize transcription factors
- Custom screening against a human transcription factor collection
Three collections of transcription factors are available:
i. A complete collection of 2,000 Arabidopsis transcription factors (Pruneda-Paz, Breton et al. 2014; Gaudinier, Zhang et al. 2011; Li, Gaudinier et al. 2014) TFLIST 11-30-2015 (full Arabidopsis TF collection);
ii. A collection of 500 maize transcription factors (Burdo, Gray et al. 2014) Maize TF Master list 3-2016;
iii. A collection of 182 Sorghum transcription factors (expressed in the root with enrichment in the vasculature). Original open reading frames synthesized as part of a DOE JGI CSP call (Sorghum TF list).
The user will recombine their promoter(s) into a Gateway 5’TOPO plasmid (or Gateway pDONR-P4P1 plasmid if available) followed by the pMW2 and pMW3 yeast reporter plasmids. These constructs will then be provided to the UC Davis Yeast One Hybrid Services Core. The UC Davis Yeast One Hybrid Services Core completes yeast transformation, auto-activation tests, genotyping, and mating-based screens. Positive interactions are recorded for LacZ and HIS3 reporters.
The Yeast One Hybrid Service Core is located within the Brady Lab.
Set up a PPMS account here (instructions here)
|Service||University of California||Non-Profit/ Education||For-Profit/ Industry|
|Screening of 1-6 Promoters/Arabidopsis 2000||$15,577||$24,845||$30,453|
|Screening of 1-24 Promoters/Maize 500||$14,825||$23,645||$28,982|
|Screening of 1-35 Promoters/Sorghum 182||$12,361||$19,715||
|Other Services (Consumables)||$1||$1||$1|
Burdo, B., J. Gray, M. P. Goetting-Minesky, B. Wittler, M. Hunt, T. Li, D. Velliquette, J. Thomas, I. Gentzel, M. dos Santos Brito, M. K. Mejia-Guerra, L. N. Connolly, D. Qaisi, W. Li, M. I. Casas, A. I. Doseff and E. Grotewold (2014). “The Maize TFome–development of a transcription factor open reading frame collection for functional genomics.” Plant J 80(2): 356-366.
Gaudinier, A., L. Zhang, J. S. Reece-Hoyes, M. Taylor-Teeples, L. Pu, Z. Liu, G. Breton, J. L. Pruneda-Paz, D. Kim, S. A. Kay, A. J. Walhout, D. Ware and S. M. Brady (2011). “Enhanced Y1H assays for Arabidopsis.” Nat Methods 8(12): 1053-1055.
Li, B., A. Gaudinier, M. Tang, M. Taylor-Teeples, N. T. Nham, C. Ghaffari, D. Benson, M. Steinmann, J. A. Gray, S. M. Brady and D. J. Kliebenstein (2014). “Promoter based integration in plant defense regulation.” Plant Physiol.
Pruneda-Paz, J. L., G. Breton, D. H. Nagel, S. E. Kang, K. Bonaldi, C. J. Doherty, S. Ravelo, M. Galli, J. R. Ecker and S. A. Kay (2014). “A genome-scale resource for the functional characterization of Arabidopsis transcription factors.” Cell Rep 8(2): 622-632.